Characterization of a rat tRNA gene cluster containing the genes for tRNAAsp, tRNAGlyand tRNAGhi, and pseudogenes
- 1 January 1982
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 10 (14) , 4441-4448
- https://doi.org/10.1093/nar/10.14.4441
Abstract
The putative genes for tRNAGAUAsp(C), tRNAGGAGly(G) and tRNAGAGGlu are in a cluster on the rat chromosome and are present exclusively in a 3.3 kb region cleaved with a restriction endonuclease EcoRI. The cluster reiterates about 10 times on the haploid DNA. Four lambda clones each containing an independent repeating unit were isolated from a rat gene library. The studies on the cloned DNA revealed that the length of the repeating unit including the 3.3 kb EcoRI fragment was at least 13.5 kb. Nucleotide sequence analysis of the 3.3 kb DNA in the isolated clones showed sequence variations among the repeating units and incomplete genes for tRNAGly and tRNAGlu within the clusters.Keywords
This publication has 22 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Nucleotide sequence of genes coding for tRNAPhe and tRNATyr from a repeating unit of X. laevis DNACell, 1980
- The rat serum albumin gene: analysis of cloned sequences.Proceedings of the National Academy of Sciences, 1979
- Cloning of Yeast Transfer RNA Genes in Escherichia coliScience, 1977
- Base sequence complexity of the stable RNA species of Drosophila melanogasterBiochemistry, 1976
- Arrangement of transfer-RNA genes in yeastNucleic Acids Research, 1976
- Low molecular weight RNA species from chromatinBiochemistry, 1975
- Reiterated transfer RNA genes of Xenopus laevisJournal of Molecular Biology, 1973
- Kinetic complexity of RNA moleculesJournal of Molecular Biology, 1972
- Proportion of the HeLa cell genome complementary to transfer RNA and 5 s RNAJournal of Molecular Biology, 1971