Triglyceride Lipase Activity in Rhesus Monkey Spermatozoa
- 1 December 1974
- journal article
- Published by Oxford University Press (OUP) in Biology of Reproduction
- Vol. 11 (5) , 578-586
- https://doi.org/10.1095/biolreprod11.5.578
Abstract
This study reports the presence of triglyceride (TG) lipase activity in monkey spermatozoa. Sperm sonicates catalyzed the hydrolysis of [14C]triolein to [14C]oleic acid at a rate of approximately 180 nmoles fatty acid/h · 109 sperm. The rate of fatty acid release was linear with time and sperm concentration. The apparent Km of TG lipase in sperm sonicates was 3 mM triolein. Subcellular fractionation of sperm sonicates showed 59, 21, and 14% of the total cellular activity associated with a l00,000g soluble fraction, a 3000g pellet, and a 300g head fraction, respectively.Keywords
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