Deoxyribonucleic acid (DNA) damage induced by bleomycin-Fe(II) in vitro: Formation of 8-hydroxyguanine residues in DNA.
- 1 January 1989
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 37 (4) , 1028-1030
- https://doi.org/10.1248/cpb.37.1028
Abstract
Treatment of calf thymus deoxyribonucleic acid (DNA) with bleomycin-Fe(II) at 0.degree. C for 5 min resulted in the formation of 8-hydroxyguanine (8-OH-Gua) residues in DNA in a dose dependent manner, in addition to the formation of base propenal, a DNA-degradation product. The amount of 8-OH-Gua was about one-hundredth of that of base propenal. Treatment of cellular DNA with bleomycin did not result in any increase of 8-OH-Gua even under conditions of 100% cell killing.This publication has 5 references indexed in Scilit:
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