UREASE ACTIVITY IN SUBTROPICAL, ALKALINE SOILS OF INDIA

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Abstract
Urea (200 .mu.g urea-N/g soil) was completely hydrolyzed within 20-56 h in alkaline, subtropical soils of India. Michaelis constant (Km) values ranged 2.0-32.0 .times. 10-3 M, and urease activity (.mu.g urea-N hydrolyzed/g soil per h) ranged 3.5-10.0. Urease activity was correlated significantly with organic C (r[correlation coefficient] = 0.8), but was not significantly correlated with clay (r = 0.45) and pH (r = -0.54). Added urease was deactivated after 96 h of incubation. High pH and free ammonia, resulting from rapid urea hydrolysis, caused accumulation and persistence of nitrites in soils receiving high rates of urea.

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