Simple Free Amino Acid Separation by Reversed-Phase Ion-Pair Liquid Chromatography Using Column Switching Technique
- 1 June 1988
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 11 (8) , 1741-1751
- https://doi.org/10.1080/01483918808076734
Abstract
Reversed-phase ion-pair liquid chromatography with copper (II) ions (RP-IPC-Cu) was applied to develop a high speed separation of free amino acids. Dynamic gradient elution in RP-IPC-Cu could not achieve this purpose due to base line shift, therefore a column switching technique was used with a combination of different alkyl-bonded silica gel columns under isocratic elution. The flexibility of eluent components makes it easy to realize a rapid separation of a mixture of targeted amino acids.Keywords
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