10-MINUTE SILVER STAIN FOR PNEUMOCYSTIS-CARINII AND FUNGI IN TISSUE-SECTIONS

  • 1 January 1982
    • journal article
    • research article
    • Vol. 106  (6) , 292-294
Abstract
A 10 min methenamine silver staining procedure for the rapid demonstration of P. carinii and fungi in tissue sections has been developed. The procedure is optimal for both urgent and routine histologic diagnosis of diffuse, progressive and life-threatening pulmonary infiltrates, especially in the compromised host, and of fungal lesions in general. Moreover, it is simple, brief, applicable to both paraffined and frozen sections, and can easily be used by the pathology resident or pathologist if the need arises. It consistently yields results that are far superior to those obtained by Grocott''s original methenamine silver method, and as good as or superior to those of modified silver methods and such nonsilver procedures as Giemsa''s, Gram-Weiger and toluidine blue stains.

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