G protein mRNA mapped in rat brain by in situ hybridization.
- 1 April 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (8) , 2864-2868
- https://doi.org/10.1073/pnas.85.8.2864
Abstract
Guanine nucleotide-binding regulatory proteins (G proteins) mediate many receptor-coupled signal transduction events. We have localized in rat brain by in situ hybridization the mRNA for the G protein subunits.sbd.G.alpha.s, G.alpha.o and G.beta.. Oligonucleotide probes were radiolabeled by a technique that resulted in a probe of defined specific activity and uniform length. mRNA species encoding g.alpha.s and G.beta. occur in high densities heterogeneously throughout the brain, especially in large neuronal cell bodies.sbd.e.g., hippocampal pyramidal cells, granule cells of the dentate gyrus, hypothalamic nuclei, and neurons of brainstem nuclei and the reticular formation. G.alpha.o mRNa has a more limited distribution and abundance, being detectable in the claustrum, endopiriform nucleus, habenula, hippocampal pyramidal cells, granule cells of the dentate gyrus, and cerebellar Purkinje cells.This publication has 22 references indexed in Scilit:
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