Purification of human pituitary LH and thyrotrophin by hydrophobic chromatography
- 1 June 1990
- journal article
- research article
- Published by Bioscientifica in Journal of Endocrinology
- Vol. 125 (3) , 493-500
- https://doi.org/10.1677/joe.0.1250493
Abstract
A new preparative procedure is described for the efficient purification of LH and TSH from frozen human pituitary glands. LH and TSH were isolated simultaneously from crude preparations by hydrophobic-interaction chromatography followed by separation and purification by reverse-phase high-performance liquid chromatography in a single step. Highly purified hormones were prepared in good yields (45 mg LH and 20 mg TSH/1000 glands) and with high biological activities; the potencies of purified LH and TSH were 5·8 × NIH-LH-S1 equivalent in an ovarian ascorbic acid depletion assay and 7·1 U/mg against human TSH MRC Research Standard (T1 70/9) in the McKenzie assay respectively. Cross-contamination by other glycoprotein hormones was low. Journal of Endocrinology (1990) 125, 493–500This publication has 10 references indexed in Scilit:
- Asparagine-linked oligosaccharides on lutropin, follitropin, and thyrotropin. II. Distributions of sulfated and sialylated oligosaccharides on bovine, ovine, and human pituitary glycoprotein hormones.Journal of Biological Chemistry, 1988
- Influence of quaternary structure on glycosylation. Differential subunit association affects the site-specific glycosylation of the common beta-chain from Mac-1 and LFA-1.Journal of Biological Chemistry, 1986
- Median charge and charge heterogeneity of human pituitary FSH, LH and TSHActa Endocrinologica, 1985
- Rapid and Easy Separation of the Subunits of Bovine and Human Glycoprotein Hormones by Use of High Performance Liquid Chromatography*Endocrinology, 1984
- Reversed-phase high-performance liquid chromatography of human thyroid-stimulating hormoneJournal of Chromatography A, 1983
- A comparison of preparations of highly purified human pituitary luteinizing hormone: differences in the luteinizing hormone potencies as determined by in vivo bioassays, in vitro bioassay and immunoassayActa Endocrinologica, 1982
- Molecular composition of human luteinizing hormone: biological and immunological profiles of highly purified preparations after electrofocusingJournal of Endocrinology, 1982
- The Isolation of Human Pituitary Hormones from Frozen Glands*Journal of Clinical Endocrinology & Metabolism, 1981
- Urinary FSH and LH Excretion During the Normal Menstrual CycleJournal of Clinical Endocrinology & Metabolism, 1964
- THE BIOASSAY OF THYROTROPIN IN SERUM1Endocrinology, 1958