Hyperthermic Enhancement of Radiation Cell Killing in HeLa S3 Cells and Its Effect on the Production and Repair of DNA Strand Breaks

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Abstract
Cell killing and the induction and repair of DNA strand breaks were examined after hyperthermia and electron irradiation treatments of HeLa S3 [human carcinoma] cells. Only heat did not produce detectable DNA strand breaks. Preirradiation heat treatment did not alter the initial levels of radiation-induced DNA strand breaks. The subsequent rate and extent of DNA strand break repair were significantly reduced by preirradiation heat treatments of 44.degree. C for 30 or 60 min. A heat treatment of 44.degree. C for 10 min, which produced no measurable cell kill alone, reduced the rate of DNA strand break repair, but the residual level of DNA strand breakage was not significantly different from unheated controls. When a time gap of up to 1 h was introduced between heating and irradiation there was no significant reduction of the thermal enhancement effect for cell killing or recovery from the reduced ability to repair DNA strand breaks.

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