Denervation Supersensitivity of the Rat Pineal to Norepinephrine‐Stimulated [3H]Inositide Turnover Revealed by Lithium and a Convenient Procedure

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Abstract
A convenient procedure for the assay of myo-[2-3H(N)]inositol ([3H]inositol) metabolites in cells or small amounts of tissue was developed. The procedure is a composite of modifications of published methods. After preincubation with [3H]inositol, rat pineal glands were disrupted in an acidified organic solvent mixture. Lipids were separated from the hydrophilic products and precursor using Sephadex G-25 columns and further analyzed by TLC. Hydrophilic products were further analyzed by anion-exchange column chromatography using Dowex AG1-X8 (formate form). In the presence of Li, increases in inositol phosphates consequent to stimulation of the glands by norepinephrine were apparent within 10 min. The response in denervated glands was considerably greater than in intact pineals.