G TO A SUBSTITUTION IN THE PROMOTER REGION OF THE APOLIPOPROTEIN-A-I GENE IS ASSOCIATED WITH ELEVATED SERUM APOLIPOPROTEIN-A-I AND HIGH-DENSITY-LIPOPROTEIN CHOLESTEROL CONCENTRATIONS

  • 1 June 1990
    • journal article
    • research article
    • Vol. 7  (3) , 233-241
Abstract
We have determined the sequence of 250 bases 5'' of the transcriptional start site of the apolipoprotein AI gene in a human individual with high serum concentrations of apo AI. One of the alleles contained a G to A substitution at position -75, between the CACAT sequence and the TAAATA box, creating a tandem repeat, CAGGGC-CA*GGGC. The substitution destroys an MspI cutting site, and the polymerase chain reaction and MspI digestion were used to identify the presence of the A or G base. The frequency of the A substitution in 96 healthy men from Bristol was 0.11 and this was increased to 0.25 in men with serum apo AI concentrations greater than 180 mg/dl. Mean with the A allele had significantly higher serum concentrations of apo AI, high density lipoprotein (HDL) cholesterol and HDL2 than those with the G allele. In this sample, variation associated with the G to A substitution accounted for 6% and 4.6% of the total variance in apo AI and HDL cholesterol concentrations, respectively. Although there is as yet no functional proof, it is possible that the A substitution may be having a direct positive effect on the rate of apo AI gene transcription and thus be associated with increased apo AI and HDL cholesterol concentrations because of increased production of apo AI protein from the liver or intestine.