Methionine acts as a “magnet” in photoaffinity crosslinking experiments
- 28 February 2006
- journal article
- Published by Wiley in FEBS Letters
- Vol. 580 (7) , 1872-1876
- https://doi.org/10.1016/j.febslet.2006.02.050
Abstract
Photoaffinity crosslinking has been utilized to probe the nature of the ligand–receptor interface for a number of G protein‐coupled receptor systems. Often the photoreactive benzophenone moiety incorporated in the ligand is found to react with a methionine in the receptor. We introduced methionines one‐at‐a‐time into the region 163–176 of the parathyroid hormone receptor, and find that crosslinking occurs to the side‐chain of methionine over a range of 11 amino acids. We call this the “Magnet Effect” of methionine. Hence, crosslinking contact points can be significantly shifted by the presence of methionine in a receptor domain.Keywords
This publication has 21 references indexed in Scilit:
- The Mid-Region of Parathyroid Hormone (1−34) Serves as a Functional Docking Domain in Receptor ActivationBiochemistry, 2006
- Determining the Environment of the Ligand Binding Pocket of the Human Angiotensin II Type I (hAT1) Receptor Using the Methionine Proximity AssayPublished by Elsevier ,2005
- CγH2 of Met174 Side Chain Is the Site of Covalent Attachment of a Substance P Analog Photoactivable in Position 5Journal of Biological Chemistry, 2002
- Involvement of the Second Extracellular Loop (E2) of the Neurokinin-1 Receptor in the Binding of Substance PJournal of Biological Chemistry, 2002
- Molecular Characterization of the Substance P·Neurokinin-1 Receptor ComplexPublished by Elsevier ,2001
- Evidence for Spatial Proximity of Two Distinct Receptor Regions in the Substance P (SP)·Neurokinin-1 Receptor (NK-1R) Complex Obtained by Photolabeling the NK-1R withp-Benzoylphenylalanine3-SPPublished by Elsevier ,2001
- Further Definition of the Substance P (SP)/Neurokinin-1 Receptor ComplexPublished by Elsevier ,2001
- Binding Domain of Human Parathyroid Hormone Receptor: From Conformation to Function,Biochemistry, 1998
- Identification of Methionine as the Site of Covalent Attachment of a p-Benzoyl-Phenylalanine-containing Analogue of Substance P on the Substance P (NK-1) ReceptorPublished by Elsevier ,1996
- Probing the Bimolecular Interactions of Parathyroid Hormone with the Human Parathyroid Hormone/Parathyroid Hormone-Related Protein Receptor. 1. Design, Synthesis and Characterization of Photoreactive Benzophenone-Containing Analogs of Parathyroid HormoneBiochemistry, 1995