Genome Replication and Progeny Virion Production of Herpes Simplex Virus Type 1 Mutants with Temperature-Sensitive Lesions in the Origin-Binding Protein
Open Access
- 1 June 2005
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 79 (11) , 7273-8
- https://doi.org/10.1128/jvi.79.11.7273-7278.2005
Abstract
Genome replication of herpes simplex viruses (HSV) in cultured cells is thought to be started by the action of the virus-encoded origin-binding protein (OBP). In experiments using two HSV-1 mutants with temperature-sensitive lesions in the helicase domain of OBP, we demonstrated that this function is essential during the first 6 hours of the lytic cycle. Once DNA synthesis has started, this function is no longer required, suggesting that origin-driven initiation of viral DNA replication is a single event rather than a continuous process.Keywords
This publication has 36 references indexed in Scilit:
- Catalysis of Strand Exchange by the HSV-1 UL12 and ICP8 Proteins: Potent ICP8 Recombinase Activity is Revealed upon Resection of dsDNA Substrate by NucleasePublished by Elsevier ,2004
- Functional Interaction between the Herpes Simplex Virus Type 1 Polymerase Processivity Factor and Origin-Binding Proteins: Enhancement of UL9 Helicase ActivityJournal of Virology, 2003
- Existence of Transdominant and Potentiating Mutants of UL9, the Herpes Simplex Virus Type 1 Origin-Binding Protein, Suggests that Levels of UL9 Protein May Be Regulated during InfectionJournal of Virology, 2003
- Residues within the Conserved Helicase Motifs of UL9, the Origin-binding Protein of Herpes Simplex Virus-1, Are Essential for Helicase Activity but Not for Dimerization or Origin Binding ActivityJournal of Biological Chemistry, 2001
- Phosphorylation of the Herpes Simplex Virus Type 1 Origin Binding ProteinJournal of Virology, 2001
- Thermosensitive UL9 gene function is required for early stages of herpes simplex virus type 1 DNA synthesisJournal of General Virology, 1995
- The herpes simplex virus type 1 origin-binding protein interacts specifically with the viral UL8 proteinJournal of General Virology, 1994
- Physical Mapping of Temperature-sensitive Mutations of Herpes Simplex Virus Type 1 Using Cloned Restriction Endonuclease FragmentsJournal of General Virology, 1983
- Control of Protein Synthesis in Herpesvirus-infected Cells: Analysis of the Polypeptides Induced by Wild Type and Sixteen Temperature-sensitive Mutants of HSV strain 17Journal of General Virology, 1976
- Genetic Studies with Herpes Simplex Virus Type 1. The Isolation of Temperature-sensitive Mutants, their Arrangement into Complementation Groups and Recombination Analysis Leading to a Linkage MapJournal of General Virology, 1973