A COMPARISON OF CLOSTRIDIAL FERREDOXINS

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Abstract
Ferredoxin occurs in Clostridium tetano-morphum, Methanobacterium omelianskii, C. acidi-urici, C. cylindro-sporum, C. pasteurianum, C. kluyveri, and C. butyricum. It does not occur in aerobic or facultatively anaerobic microorganisms or in beef liver. A method is described for the purification and crystallization of the protein from C. acidi-urici. Purified preparations from C. acidi-urici, C. pasteurianum, and C. butyricum have essentially the same enzymic activity, an absorption maximum at 390 m[mu] and approximately 1 [mu]mole of "labile sulfide" and non-heme Fe/mg. These preparations differ in their UV and infrared absorption spectra and amino-acid composition. Crystalline preparations from C. acidi-urici and C. pasteurianum lack methionine, histidine, tryptophan and leucine, and contain only a single basic amino-acid. The ferredoxin of C. acidi-urici contains arginine but no lysine or phenylalanine, whereas C. pasteurianum ferredoxin contains lysine and no arginine.