Genetic Map of Bacteriophage φX174
Open Access
- 1 May 1971
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 7 (5) , 549-558
- https://doi.org/10.1128/jvi.7.5.549-558.1971
Abstract
Bacteriophage φX174 temperature-sensitive and nonsense mutations in eight cistrons were mapped by using two-, three-, and four-factor genetic crosses. The genetic map is circular with a total length of 24 × 10 −4 wt recombinants per progeny phage. The cistron order is D-E-F-G-H-A-B-C. High negative interference is seen, consistent with a small closed circular deoxyribonucleic acid molecule as a genome.Keywords
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