DNA synthesis by partially purified replicating simian virus 40 chromosomes

Open Access

1 September 1977

journal article
research article
Published by Oxford University Press (OUP) in Nucleic Acids Research

Vol. 4 (9) , 3083-3096
https://doi.org/10.1093/nar/4.9.3083

Abstract

We have partially purified replicating simian virus 40 (SV40) chromosomes in a form which allows continued DNA synthesis in vitro. We first prepare a soluble DNA-synthesizing system from SV40-infected monkey cells and then sediment the components through a neutral sucrose gradient of extremely low ionic strength. Replicating SV40 chromosomes isolated from such gradients are capable of continuing DNA synthesis in vitro in the same manner as two crude subnuclear systems we have previously described (4). This indicates that the enzymes and other proteins required for in vitro DNA synthesis are bound to the replicating chromosomes.

Keywords

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