Cryoglobulinemia: Analysis of isotype, idiotype and antibody activity by composite gel electrophoresis and immunoblotting

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Abstract
Improved methods for high resolution composite gel electrophoresis under dissociating conditions and electrophoretic transfer of immunoglobulins (Mr 150 000-1 800 000) to nitrocellulose have been developed. Using these techniques and highly specific antisera to detect either light or heavy chains on nitrocellulose transfers, the immunoglobulin and clonal composition of washed cryoglobulins (3 μg each) could be determined within two days. The results were confirmed by isoelectric focusing and immunoblotting of selected samples. In addition, the method was used for detection of andiotypic determinant and rheumatoid factor activity within components of the cryoglobulins.