Haemoglobin binding with haptoglobin. Localization of the haptoglobin-binding site on the α-chain of human haemoglobin
- 1 August 1981
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 197 (2) , 507-510
- https://doi.org/10.1042/bj1970507
Abstract
A synthetic approach was employed to identify the haptoglobin-binding site on the .alpha.-chain of human Hb. This approach consists of the synthesis of a series of consecutive overlapping peptides that, together, systematically represent the entire protein chain. Fourteen peptides were synthesized (.alpha.1-15, .alpha.11-25, .alpha.21-35, .alpha.31-45, .alpha.41-55, .alpha.51-65, .alpha.61-75, .alpha.71-85, .alpha.81-95, .alpha.91-105, .alpha.101-115, .alpha.111-125, .alpha.121-135 and .alpha.131-141), and their ability to bind human haptoglobin was studied. Only peptide .alpha.121-135 bound haptoglobin significantly. The haptoglobin-binding site on the .alpha.-chain of Hb resides within, but does not necessarily encompass all of, the region .alpha.121-135.This publication has 21 references indexed in Scilit:
- The antibody response to myoglobin is independent of the immunized species. Analysis in terms of replacements in the antigenic sites and in environmental residues of the cross-reactions of fifteen myoglobins with sperm-whale myoglobin antisera raised in different speciesBiochemical Journal, 1980
- Antibody combining sites can be mimicked synthetically. Surface-simulation synthesis of the phosphorylcholine-combining site of myeloma protein M-603Biochemical Journal, 1980
- Distance calculation of residues neighbouring to lysozyme antigenic sites. Site-neighbouring residues whose evolutionary substitution can modify the characteristics and binding energy of the sitesBiochemical Journal, 1980
- Binding of Human Hemoglobin and Its Polypeptide Chains with Haptoglobin Coupled to an Agarose MatrixEuropean Journal of Biochemistry, 1976
- Three-dimensional fourier synthesis of human deoxyhaemoglobin at 2·5 Å resolution: Refinement of the atomic modelJournal of Molecular Biology, 1975
- The Binding of Hemoglobin to Haptoglobin and Its Relation to Subunit Dissociation of HemoglobinJournal of Biological Chemistry, 1971
- Three-dimensional Fourier Synthesis of Horse Oxyhaemoglobin at 2.8 Å Resolution: The Atomic ModelNature, 1968
- Studies on the reaction of haptoglobin with haemoglobin and haemoglobin chainsJournal of Molecular Biology, 1968
- STRUCTURE OF HUMAN HEMOGLOBIN .4. CHYMOTRYPTIC DIGESTION OF ALPHA CHAIN OF HUMAN HEMOGLOBIN1962
- Die Konstitution des normalen adulten HumanhämoglobinsHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1961