Proviral DNA of Moloney Leukemia Virus: Purification and Visualization
- 13 February 1976
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 191 (4227) , 569-571
- https://doi.org/10.1126/science.1251192
Abstract
Closed-circular proviral DNA of Moloney leukemia virus has been purified from a 10(7) excess of cellular and mitochondrial DNA. The DNA can be visualized in the electron microscope and has the contour length of a molecule with a moleculecular weight of about 5.5 + 10(6). Electron microscopic observation of a hybrid between viral RNA and this circular DNA confirms the viral origin of this molecule.Keywords
This publication has 15 references indexed in Scilit:
- Selective extraction of polyoma DNA from infected mouse cell culturesPublished by Elsevier ,2004
- Ethidium bromide inhibits appearance of closed circular viral DNA and integration of virus-specific DNA in duck cells infected by avian sarcoma virusNature, 1975
- Three abundance classes in HeLa cell messenger RNANature, 1974
- [31] Formation and visualization of rings from eukaryotic DNA fragmentsPublished by Elsevier ,1974
- Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose-ethidium bromide electrophoresisBiochemistry, 1973
- Ribonucleotides in closed circular mitochondrial DNA from HeLa cellsBiochemical and Biophysical Research Communications, 1973
- RNase and alkali sensitivity of closed circular mitochondrial DNA of rat ascites hepatoma cellsBiochemical and Biophysical Research Communications, 1973
- Small polydisperse circular DNA of HeLa cellsJournal of Molecular Biology, 1972
- A physical study by electron microscopy of the terminally repetitious, circularly permuted DNA from the coliphage particles of Escherichia coli 15Journal of Molecular Biology, 1970
- A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.Proceedings of the National Academy of Sciences, 1967