Fluorometric Determination of Diarrhetic Shellfish Toxins by High-Performance Liquid Chromatography

1 March 1987

journal article
research article
Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry

Vol. 51 (3) , 877-881
https://doi.org/10.1080/00021369.1987.10868078

Abstract

Two principal toxins of diarrhetic shellfish poisoning, okadaic acid and dinophysistoxin-1, were esterified with 9-anthryldiazomethane in methanol. After cleaning with a Sep-pak silica cartridge column, the fluorescent esters were analyzed on a Develosil ODS column with MeCN- MeOH-H₂0 (8:1:1). The fluorescence intensities of both toxin derivatives measured at an excitation of 365 nm and an emission of 412 nm showed good linearity in the range 1 ~ 80ng.

This publication has 2 references indexed in Scilit:

Histopathological studies on experimental marine toxin poisoning. I. Ultrastructural changes in the small intestine and liver of suckling mice induced by dinophysistoxin-1 and pectenotoxin-1
Toxicon, 1986
Diarrhetic shellfish toxins
Tetrahedron, 1985