Transfer and Expression of Pseudomonas Plasmid RP1 in Caulobacter

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Abstract
The host range of Pseudomonas plasmid RP1 includes the genus Caulobacter. Caulobacter required 3 antibiotic resistance markers located in RP1. A 4th plasmid marker, susceptibility to an RNA bacteriophage, was not expressed, but could be transferred from Caulobacter to Escherichia coli. The lack of phenotypic expression of the phage marker was manifested by the inability of the phage to adsorb or to produce plaques on Caulobacter transcipients. Matings of P. aeruginosa and C. vibrioides cv6 were carried out in the presence of bacteriophage .phi.6, a DNA phage that infects and kills only swarmer cells of Caulobacter. No decrease in plasmid transfer in the presence of phage .phi.6 was detected, suggesting that stalked cells, and not swarmer cells, serve as recipients. Transfer of chromosomal segments from Caulobacter may be mediated by plasmid RP1; such segments are not stably maintained.

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