COMPARATIVE STUDIES ON HYDROLYSIS OF CONJUGATED ESTROGENS IN HUMAN PREGNANCY URINE

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Abstract
A method is presented for the simultaneous use of Mylase P and beta-glucuronidase for the hydrolysis of conjugated estrogens in pregnancy urine. The method yields values which compare favorably with those obtained by use of the enzymes separately in either sequence. Evidence is presented which indicates that a small amount of estriol is excreted as a double conjugate with H2SO4 and glucuronic acid. Previous comparative studies on hydrolysis of conjugated estrogens in pregnancy urine were extended to include hydrolysis with HC1 in the presence of Zn. This method yields somewhat higher values than are obtained with HC1 alone, but not appreciably higher than obtained by enzymatic hydrolysis. A comparison of the principal methods of hydrolysis using whole urines and their butanol extracts indicated that somewhat higher values are obtained with the butanol extracts by enzymatic and HC1 hydrolysis, but not by Zn-HCl.