ADAPTIVE DIFFERENTIATION OF MURINE LYMPHOCYTES .3. LYMPHOCYTES-T AND LYMPHOCYTES-B DISPLAY RECIPROCAL PREFERENCE FOR ONE ANOTHER TO DEVELOP OPTIMAL INTERACTING PARTNER CELL SETS

  • 1 January 1979
    • journal article
    • research article
    • Vol. 122  (5) , 1937-1942
Abstract
Responses to the synthetic terpolymer L-glutamic acid, L-lysine, L-phenylalanine (GL.PHI.) and hapten derivatives are controlled by 2 complementing H-2-linked Ir genes in the mouse. F1 hybrids derived from 2 different nonresponder strains (1 of which possesses the .alpha. and the other .beta. Ir-GL.PHI. gene) were phenotypic responders to GL.PHI. and 2,4-dinitrophenyl (DNP)-GL.PHI.. Spleen cells from DNP-GL.PHI.-primed F1 mice could adoptively transfer secondary anti-DNP antibody responses to irradiated F1 recipients that were challenged with DNP-GL.PHI.. When GL.PHI.-primed F1 helper T [thymus-derived] cells were transferred together with the DNP-specific F1 B [bone marrow-derived] cells that were primed in separate mice by DNP coupled to an unrelated protein carrier, such mixtures failed to develop adequate adoptive secondary anti-DNP responses to DNP-GL.PHI.. This contrasted with the ability of the same GL.PHI.-primed F1 T cells to provide helper activity for DNP-primed B cells from responder recombinant B10.A (5R) mice. The apparent defect of GL.PHI.-primed F1 T cells in providing help for DNP-primed F1 B cells (primed to a DNP-protein conjugate) were readily overcome by using DNP-primed B cells from donor F1 mice primed with DNP-GL.PHI.. Interacting T and B lymphocytes apparently pair off into partner cell sets, any pair of which interact optimally when a best fit reciprocal self-recognition occurs between them.

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