EDTA effect on creatine kinase (CK) and on the SCE reagent
- 1 December 1979
- journal article
- research article
- Published by Taylor & Francis in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 39 (8) , 737-742
- https://doi.org/10.3109/00365517909108165
Abstract
The inclusion of EDTA in the creatine kinase reagent recommended by the Scandinavian Committee on Enzymes was shown to increase reagent stability from less than 24 h to 5 days. Part of this effect can be explained by the fact that EDTA delays the formation of inhibitory products formed when N-acetyl cysteine is oxidized. The addition of EDTA to the reagent also results in increased measured CK activity. This effect is more pronounced for CK-BB than for CK-MM. Calcium and ferric ions are shown to inhibit the enzyme and the chelation of these ions can partly explain the observed increase of CK activity.Keywords
This publication has 1 reference indexed in Scilit:
- Effects of β-mercaptoethanol and chelating agents on the stability and activation of creatine kinase in serumClinica Chimica Acta; International Journal of Clinical Chemistry, 1978