Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content.
Open Access
- 1 July 1996
- journal article
- Published by Cold Spring Harbor Laboratory in Genome Research
- Vol. 6 (7) , 633-638
- https://doi.org/10.1101/gr.6.7.633
Abstract
A PCR method for uniform amplification of a mixture of DNA templates differing in GC content is described using the two enzyme approach (Klentaq1 and Pfu DNA polymerase) and a combination of DMSO and betaine. This method was applied to amplify the CGG repeat region from the fragile X region.Keywords
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