Stability of the endo-β-1, 4-glucanase and β-1, 4-glucosidase from Bacteroides succinogenes
- 1 January 1982
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 28 (1) , 144-148
- https://doi.org/10.1139/m82-017
Abstract
The endo-.beta.-1,4-glucanase (carboxymethylcellulase) activity in cell extracts prepared from B. succinogenes S85 was almost unaffected by prolonged incubation at 39.degree. C in the presence of merthiolate, a SH inhibitor. The .beta.-1,4-glucosidase (cellobiase) activity was rapidly inactivated by the same treatment. The cellobiase also was inactivated by exposure to air but was stabilized by dithiothreitol in a N2 atmosphere. The cellobiase apparently required reduced SH groups for activity.This publication has 2 references indexed in Scilit:
- Intracellular Distribution and Some Properties ofβ-Glucosidases of a Cellulolytic PseudomonadAgricultural and Biological Chemistry, 1976
- Measurement of carboxymethylcellulase activityAnalytical Biochemistry, 1960