A rapid method for measuring blood oxygen content utilizing the oxygen electrode.

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Abstract
A method is described for measuring blood oxygen content by determining the amount of physically dissolved oxygen released from hemoglobin by ferricyanide with a membrane-covered oxygen electrode. Degassed ferricyanide solution is allowed to run through a stopcock bore containing the blood sample (0. 12 ml) into a syringe where mixing is accomplished. The oxygen content of the blood sample is calculated from the increase in oxygen tension measured in the blood-ferricyanide mixture. With simultaneous determinations of blood oxygen content by the Van Slyke method and the electrode technique, the average discrepancy is 3.2% (0. 37 ml/100 ml) with the standard deviation of the difference being 4. 0% (0. 5 ml/100 ml). One determination can be done in 6 or 7 min and a high degree of reproducibility attained with small demand on technical facility.

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