Monitoring frequencies of insecticide resistance in Myzus persicae (Sulzer) (Hemiptera: Aphididae) in England during 1985–86 by immunoassay

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Abstract
An immunoassay for the carboxylesterase E4, which confers insecticide resistance in Myzus persicae (Sulzer), was used to monitor the distributions of E4 activity within samples comprising more than 5000 aphids from sites in eastern and southern England. The quantitative precision of this assay is an improvement on the subjective assessment of E4 band intensity on electrophoresis gels previously used. Activity distributions did not always show discrete components corresponding to S, R1, R2 or R3 variants and thus, though convenient, this classification should be used cautiously. Distributions derived by immunoassay of many insects from the field are best compared to those of laboratory-reared standards classified according to E4 quantity (V1-V64). Data from untreated crops are presented in this way and discussed in the light of previous surveys. A large proportion of aphids corresponding to the V8 variant (intermediate between ‘R1’ and ‘R2’ categories) were found, and frequencies of resistance differed on both a local and regional scale. The majority of aphids from treated crops had E4 activities equivalent to, or above, that of V16 (i.e. ‘R2’).

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