Calcium sensitivity of microtubles changes during the cell cycle of Xenopus laevis tadpole endothelial cells
- 1 April 1988
- journal article
- research article
- Published by Wiley in Cell Biology International Reports
- Vol. 12 (4) , 313-320
- https://doi.org/10.1016/0309-1651(88)90076-8
Abstract
Endothelial cells derived from Xenopus tadpole hearts (XTH-2: Schlage et al. 1981) have been permeabilized for calcium using the ionophore A 23187. A Ca2+-concentration of 1 .mu.M causes a graded disassembly of microtubules (mts); with increasing periods of incubation mts desintegrate and then depolymerize completely. The frequency of the different stages of depolymerization depends on the length of the Ca2+ incubation period, even after 60 min incubation time some mts remain. The stage of depolymerization of mts in a cell is correlated with its cell cycle phase showing the highest stability during S phase.This publication has 1 reference indexed in Scilit:
- Carboxyl terminal tyrosine metabolism of alpha tubulin and changes in cell shape: Chinese hamster ovary cellsBiochemical and Biophysical Research Communications, 1981