The uptake of tritiated thymidine by human amnion cells: a correlation with cell structure

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Abstract
It was found that less than 1% of amnion cells, either as membrane biopsies, as trypsinized cell suspensions, or grown as monolayers on glass incorporated tritiated thymidine during a 1-hour incubation period; this was in contrast to HeLa cells in which over 30% of the cell population incorporated tritiated thymidine. Continuous labelling with tritiated thymidine for a 24-hour period with or without colchicine failed to increase the percentage of labelled amnion cells, while a minimum of 80% of the HeLa cells became labelled. Less than 0.1% of the amnion cells were in mitosis, compared to 4% for HeLa cells. Incubation with colchicine for 24 hours failed to increase the percentage of mitotic figures of the amnion cells. It is concluded that most of the primary amnion cells are in the non-proliferative phase of the cell cycle.

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