OVARIAN BIOCHEMICAL COMPETENCE FOLLOWING GONADOTROPHIC DEPRIVATION FROM BIRTH

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Abstract
Newborn mice were treated daily with an antiserum neutralizing endogenous circulating gonadotropins. At the age of 14 days, ovaries from these mice and control littermates were incubated in the presence and absence of gonadotrophic preparations; cAMP [cyclic AMP] and lactic acid levels were then measured in tissue and incubation medium. Ovaries from antigonadotropin treated and control mide had the same basal levels, per mg tissue, of cAMP and lactic acid. The levels increased to approximately the same extent following in vitro gonadotropic stimulation: an increase in cAMP of 8-10-fold (using a preparation with an hLH:hFSH [human luteinizing hormone: human follicle stimulating hormone] ratio of 1:1) or 21/2-fold (using a preparation with an hLH:hFSH ratio of 1:5), and about a 2-fold increase in lactic acid (using oLH [ovine LH]). Despite impaired morphogenesis, the enzymatic systems necessary for ovarian glycolysis (as measured by lactic acid production) and cAMP formation can develop without gonadotropic participation. Ovarian capacity to respond to hormonal stimulation is acquired post-natally, as shown by refractoriness to gonadotropic stimulation during the 1st wk of life. Post-natal gonadotropic exposure does not seem an essential requirement, at least as measured by the above parameters, for acquiring ovarian competence to respond to hormonal stimulation.

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