KIM185, a monoclonal antibody to CD18 which induces a change in the conformation of CD18 and promotes both LFA‐1‐ and CR3‐dependent adhesion

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Abstract
Unactivated peripheral blood leukocytes show little tendency to bind to other cells or matrix components, whilst, in the presence of inflammatory mediators, adhesive interactions can rapidly increase. The Leu-CAM (β2 integrin) family of adhesion molecules have been shown to mediate a variety of these induced adhesion events. Here we describe a monoclonal antibody against CD18, KIM185, which stimulates JY cell homotypic aggregation by a CD11a pathway as well as inducing the adherence of neutrophils to protein-coated plastic by a CD lib-dependent mechanism. The antibody recognizes an epitope distinct from the previously described KIM127 antibody and evidence is presented that the binding of KIM185 can cause a change in the conformation of the CD18 molecule.