Evidence for the excision repair of O6-n-butyldeoxyguanosine in human cells
- 1 December 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 7 (12) , 1987-1990
- https://doi.org/10.1093/carcin/7.12.1987
Abstract
The persistence of O6 - n -butyldeoxyguanosine ( O6 -nBudG) in DNA, the presence of O6 -alkylguanine DNA alkyltransferase (AT) activity in cell extracts, and cell survival following exposure to N - n -butyl- N -nitrosourea (BNU), have been measured in normal and xeroderma pigmentosum cell strains, both transformed and untransformed. The rates of removal of O6 -nBudG did not correlate with AT activity but did correlate with the ability of strains to excise bulky DNA lesions. BNU and N -methyl- N -nitrosourea dose—response curves for cell killing suggests that both AT and excision may be involved in the repair of cytotoxic lesions.Keywords
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