Low‐temperature spectrophotometry of phoborhodopsin
- 29 August 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 236 (2) , 333-336
- https://doi.org/10.1016/0014-5793(88)80050-4
Abstract
Photoreactions of the fourth rhodopsin‐like pigment, phoborhodopsin (pR480), ofHalobacterium halobiumwere studied by low‐temperature spectrophotometry. Upon irradiation of pR480 at ‐ 80 as well as ‐ 170°C with 436 nm light, a new intermediate having a difference absorption maximum at about 520 nm was produced. We designate this intermediate 'P520'. Prolonged irradiation caused the formation of a photosteady‐state mixture composed of P520 and pR480, indicating that P520 was photoreversible to pR480 at ‐ 80°C. P520 was thermally converted back to pR480 through P350 and P530, both of which were detected previously [(1986) Biochem. Biophys. Res. Commun. 139, 389–395]. Based on these results, a new photochemical reaction cycle of pR is presented.Keywords
This publication has 12 references indexed in Scilit:
- Low temperature spectrophotometry on the photoreaction cycle of sensory rhodopsinFEBS Letters, 1987
- The Photochemical Cycle of HalorhodopsinBiophysical Journal, 1987
- Action spectrum of the photoattractant response of Halobacterium halobium in early logarithmic growth phase and the role of sensory rhodopsinBiochimica et Biophysica Acta (BBA) - General Subjects, 1986
- Color discrimination in halobacteria: spectroscopic characterization of a second sensory receptor covering the blue-green region of the spectrum.Proceedings of the National Academy of Sciences, 1986
- Flash spectrophotometric identification of a fourth rhodopsin-like pigment in Halobacterium halobiumBiochemical and Biophysical Research Communications, 1986
- A photosystem other than PS370 also mediates the negative phototaxis ofHalobacterium halobiumFEMS Microbiology Letters, 1985
- Absorption Spectral Properties of Purified Halorhodopsin1The Journal of Biochemistry, 1984
- Identification of a third rhodopsin-like pigment in phototactic Halobacterium halobium.Proceedings of the National Academy of Sciences, 1982
- [49] Low-temperature spectrophotometry of intermediates of rhodopsinPublished by Elsevier ,1982