Contribution of coronary endothelial cells to cardiac adenosine production

Publisher Website
Google Scholar
Abstract
Experiments were performed in isolated non-working guinea pig hearts perfused according to the Langendorff technique (95% O2, 5% CO2), to evaluate the relative contribution of the coronary endothelium to the formation of cardiac adenosine during hypoxia, hypercapnia, and acetylcholine infusion. For this purpose the adenine-nucleotides of the coronary endothelium were prelabeled by perfusion of isolated hearts with3H-adenosine (10−8 M) for 35 min. Changes in the relative specific radio-activity (RSA) of adenosine released into the coronary effluent perfusate were used to assess changes in the relative contribution of the coronary endothelium and cardiomyocytes to total cardiac adenosine release. Hypoxic perfusion (15% O2) doubled coronary flow and increased total adenosine release by about two orders of magnitude and in addition, substantially increased the release of3H-adenosine. The RSA of adenosine, however, was consistently depressed. During hypercapnic acidosis (9% CO2) the increase in coronary flow was associated with only a small and transient rise in cardiac adenosine release, and did not influence the formation of3H-adenosine. In the unpaced heart, acetylcholine (10−7 and 2×10−6 M) dose-dependently increased coronary flow and the release of both adenosine and3H-adenosine. Within the first minute, the RSA of adenosine was increased, but thereafter was decreased relative to control. In the paced heart, the effects of acetylcholine (2×10−6 M) were greatly attenuated. Increasing coronary flow by bradykinin and isosorbide dinitrate or decreasing heart rate by (−)N6-phenylisopropyl-adenosine did not significantly affect effluent perfusate concentration of adenosine or its RSA. Our findings suggest that coronary endothelium in vivo can contribute to increased cardiac adenosine release in response to hypoxia and acetylcholine but not following hypercapnic acidosis. In addition, the consistent decrease in RSA of adenosine suggests a proportionally greater increase in adenosine release from cardiomyocytes.