Influence of triiodothyronine on the amino acid uptake of brain and spinal cord in normal and spinal hemisected adult rats

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Abstract
The thyroid hormones triiodothyronine (T3) and L‐thyroxine appear to enhance regeneration in the peripheral and central nervous system (CNS). The following experiments examine possible metabolic substrates for the action of T3 on the adult rat CNS after spinal hemisection. The protein incorporation of (3H)lysine after a left spinal hemisection (T2) or control operations was examined 1, 3, 7, and 14 days postoperation. Triiodothyronine (1 μg/kg body weight in a bicarbonate buffer) was injected daily for the postoperation or equivalent time period. One hour prior to decapitation, animals were given a subcutaneous injection of 200 μCi of (3H)lysine. Samples of brain and spinal cord were dissolved, and the radioactivity of acid‐precipitable protein and acid‐soluble fractions were determined by scintillation counting. T3 treatment influenced the general levels of incorporation of all treated groups over all days postoperation. Specific effects were observed in spinal hemisected T3‐treated animals. A significant hemispheric (P3H)lysine incorporation in T3‐treated rats relative to controls. T3 effects appear to involve an increased sensitivity of the cells of the injured nervous system to the hormone.