Effect of Furfural on the In Vitro Germination ofPeronosclerospora sorghiOospores

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Abstract
Methods of inducing germination and controlling contamination in oospores of P. sorghi were evaluated. Oospores centrifuged from autolysed sorghum [Sorghum bicolor] leaf tissue had fewer contaminants than did those sieved from ground, dry tissue. Furfural, at 50-100 .mu.l/l, was effective in stimulating germination and/or suppressing contamination. In the presence of furfural, dried spores commenced germination after 3 days on agar, reached a maximum at about 5 days, but were scarcely observable after 9 days at room temperature (.apprx. 25.degree. C) because germ tubes had disintegrated or were indistinguishable from mycelia of contaminants. Washed oospores (from autolysed leaves) that were allowed to dry sometimes germinated within 2 days. Germination occurred at 16-30.degree. C and was most rapid (3-5 days) at 26-28.degree. C. Great variability in percent germination was attributed to the variability in contamination and in the proportion of mature viable oospores produced in the leaf tissue.

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