Deoxyribonucleic Acid Content of HeLa Stock and Newly Established Clone Cultures

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Abstract
The deoxyribonucleic acid (DNA) content was determined in individual cells of Feulgen-stained HeLa stock and newly established HeLa clones with a redesigned sensitive cytophotometer. The DNA content varied in cells of the stock. Similar variations in DNA were observed in clones by the time 10 divisions had occurred. Morphologic observations and cytophotometric measurements indicated that the DNA in the particular HeLa cells studied and grown under the conditions described did not reduplicate in a uniformly stable manner in populations which were started from single cells.