THE EFFECT OF CYCLOSPORIN-A ON THE ADOPTIVE TRANSFER OF EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS IN THE LEWIS RAT

  • 1 January 1982
    • journal article
    • research article
    • Vol. 47  (1) , 127-132
Abstract
Experimental allergic encephalomyelitis (EAE) can be adoptively transferred in Lewis rats with spleen cells from immunized animals after culture with concanavalin A or myelin basic protein (MBP). The effect of the immunosuppressive drug cyclosporin A (CsA) on the in vitro and in vivo steps of the cell transfer was investigated. Clinical signs of EAE were completely suppressed by CsA in rats immunized with MBP in Freund''s complete adjuvant and spleen cells from these animals, cultured with the antigen, did not transfer the disease. The incidence of transferred disease was also reduced if recipients were treated with CsA although a higher dose of drug than that needed to suppress active EAE was required. Complete suppression of EAE was only accomplished for the period of dosing although the clinical signs of disease which appeared after withdrawal of the drug were significantly reduced in severity. An immune response in the host animal apparently is a prerequisite for adoptive transfer of EAE and CsA may regulate the action of lymphocytes already primed.