Expression of a nuclear envelope protein recognized by the monoclonal antibody BU31 in lung tumours: Relationship to Ki‐67 antigen expression

Publisher Website
Google Scholar
Abstract
The production of the murine monoclonal antibody BU31 is described. This antibody identifies a nuclear envelope protein which is expressed in some but not all cells, and which resembles statin, a protein reported to be expressed by non‐proliferating cells. BU31 was applied onto frozen sections of a series of 78 lung tumours and the staining patterns were compared with those obtained with Ki‐67. There was an inverse correlation between the proportion of tumour nuclei labelled with the two reagents (r= −0.61, 95 per cent confidence intervals −0.73 to −0.45). However, the four neuroendocrine neoplasms were BU31‐negative. Squamous cell carcinomas often showed a peripheral distribution of the cells stained positively with Ki‐67, whereas BU31 tended to label centrally situated cells. These observations are consistent with the concept that the antigen recognized by BU31 is expressed by non‐proliferating cells in these tumours.