Overproduction of the Escherichia coli recA protein without stimulation of its proteolytic activity
- 1 October 1981
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 148 (1) , 386-390
- https://doi.org/10.1128/jb.148.1.386-390.1981
Abstract
Plasmid pBEU14, which carries the Escherichia coli recA+ gene and which can be amplified by manipulation of growth temperature, was constructed. When pBEU14 deoxyribonucleic acid was amplified, a high rate of synthesis and accumulation of recA protein resulted. Amplification of the recA gene and protein did not cause induction of prophage lambda, indicating that the proteolytic activity of the recA protein was not stimulated.This publication has 43 references indexed in Scilit:
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