An in vivo study of benzene metabolite DNA adduct formation in liver of male New Zealand rabbits

Abstract
Rabbits were treated with benzene (586 mg/kg/ b.i.d./4 days) after which DNA was isolated from liver and analyzed for adduct formation using the [32P] postlabeling method of Randerath and coworkers (Randerath et al. 1981; Reddy et al. 1984, 1986, 1987). Liver 500 g and 9000 g fractions were analyzed for adducts. There appeared to be several adducts in both the 500 g and 9000 g fractions observed on radioautographs of cellulose-TLC plates. Several adducts were also observed when the 9000 g fraction was studied using HPLC.