Cloning and sequence analysis of a cDNA encoding the β‐subunit of mouse β‐hexosaminidase

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Abstract
A cDNA encoding the prepro-β-polypeptide of mouse β-hexosaminidase (Hex) was isolated from a mouse lymphoblast cDNA library. The cDNA contains an open reading frame corresponding to a polypeptide of 536 amino acids which shows 74% homology with the human prepro-β-polypeptide. An examination of the amino acid sequence identifies a putative signal peptide and five possible glycosylation sites, two of which are identical to the confirmed glycosylation sites of the human β-chain. The amino acid sequence also shows a structurally similar though not identical site for internal cleavage responsible for the generation of mature βa- and βb-polypeptides.