Voltage‐dependent calcium channel β‐subunits in combination with α1 subunits, have a GTPase activating effect to promote the hydrolysis of GTP by Gαo in rat frontal cortex
Open Access
- 14 August 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 370 (1-2) , 135-140
- https://doi.org/10.1016/0014-5793(95)00813-o
Abstract
The dihydropyridine‐sensitive calcium channel agonist (−)‐BayK 8644 was found to produce an enhancement of the intrinsic hydrolysis of GTP by Go in rat frontal cortex membranes. An anti‐calcium channel β‐subunit antiserum abolished the (−)‐BayK 8644‐stimulated hydrolysis of GTP by Go and reduced the dihydropyridine binding capacity of the cortical membranes. A peptide which mimics the β‐subunit binding domain of the calcium channel complex, also attenuated (−)‐BayK 8644 activation of GTPase. This study suggests that the calcium channel β‐subunit is the principal component of the channel complex involved in linking dihydropyridine agonist binding to enhanced hydrolysis of GTP by Go. This may be a mechanism by which calcium channels can normally act to limit the duration of a G‐protein modulatory signal.Keywords
This publication has 40 references indexed in Scilit:
- Use of site‐directed antibodies to probe the topography of theα2 subunit of voltage‐gated Ca2+ channelsFEBS Letters, 1995
- Calcium channels: The β‐subunit increases the affinity of dihydropyridine and Ca2+ binding sites of the α1‐subunitFEBS Letters, 1994
- Calcium channel β-subunit binds to a conserved motif in the I–II cytoplasmic linker of the α1-subunitNature, 1994
- Potentiation by the β Subunit of the Ratio of the Ionic Current to the Charge Movement in the Cardiac Calcium ChannelScience, 1993
- Requirement of the calcium channel β subunit for functional conformationFEBS Letters, 1993
- Structure and Functional Expression of a Member of the Low Voltage-Activated Calcium Channel FamilyScience, 1993
- Characterization of β subunit modulation of a rabbit cardiac L‐type Ca2+ channel α1 subunit as expressed in mouse L cellsFEBS Letters, 1993
- 1,4‐Dihydropyridines modulate GTP hydrolysis by Go in neuronal membranesFEBS Letters, 1992
- Regulation of deactivation of photoreceptor G protein by its target enzyme and cGMPNature, 1992
- GABAB receptors couple to G proteins Go, G∗o and G·i1 but not to Gi2FEBS Letters, 1990