Ribozyme processed tRNA transcripts with unfriendly internal promoter for T7 RNA polymerase: production and activity
Open Access
- 25 September 1998
- journal article
- Published by Wiley in FEBS Letters
- Vol. 436 (1) , 99-103
- https://doi.org/10.1016/s0014-5793(98)01096-5
Abstract
A limitation for a universal use of T7 RNA polymerase for in vitro tRNA transcription lies in the nature of the often unfavorable 5′‐terminal sequence of the gene to be transcribed. To overcome this ...Keywords
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