Culture of Mesophyll Protoplasts and Stem Segments ofAntirrhinum majus(Snapdragon): Growth and Organization of Embryoids

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Abstract
Protoplasts isolated enzymatically from the leaves of Antirrhinum majus L. were cultured on a synthetic medium and their growth and development were followed in vitro. Cultured protoplasts increased in size, regenerated cell walls, divided and formed small colonies and embryoids. Cell wall formation and cell division required both auxin and cytokinin in the medium. Isolated stem segments of the same species, when cu1tured on a synthetic medium with an added auxin, yielded rapidly growing callus tissues in which differentiation of embryoids occurred.

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