Immunoassay Using a Metal-complex Compound as a Chemiluminescent Catalyst. I. Iron(III)phthalocyanine as a Labeling Reagent

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Abstract
A synthesized metal-complex compound with catalytic activity for a chemiluminescence reaction has been used for the first time as a labeling reagent for immunoassay. Several complex compounds of iron(III) and cobalt(II) were synthesized, and their catalytic activities for the chemiluminescence reaction between 5-amino-2,3-dihydro-1,4-phthalazinedione (luminol) and hydrogen peroxide (H2O2) were examined. Iron(III) 4,11,18,25-tetracarboxyphthalocyanine (TCP-Fe(III)) showed the highest catalytic activity. The immunoassay of free albumin was carried out by a competitive method using albumin labeled with TCP–Fe(III) by means of the carbodiimide method; free albumin in the range of 2 to 1000 μg/tube could be determined with a coefficient of variation of from 6 to 16% in 5 analyses.