Histamine Receptor-Bearing Leukocytes (HRL)

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Abstract
A rosette technique is described for the enumeration and identification of histamine receptor-bearing leukocytes (HRL). Mouse leukocytes from a variety of lymphoid tissues form rosettes with sheep erythrocytes coated with histaminerabbit serum albumin (RSA) conjugate. Rosette formation was specifically inhibited by either histamine-RSA or by histamine, but not by RSA. The percentage of rosettes in lymphoid tissues of adult mice ranged from 8% in the thymus to 70% in the peritoneal exudate leukocytes. Spleen, bone marrow, peripheral blood, and lymph node cells produced intermediate numbers of rosettes (20 to 40%). Spleen and peritoneal exudate leukocytes derived from mice and rats immunized against allogeneic and syngeneic tumors, respectively, demonstrated a significantly lower frequency of HRL as compared with unsensitized cells. The vast majority of immune lymphoblasts, however, exhibited a high degree of rosette formation and were coated heavily with the indicator erythrocytes. Of particular interest was the observation that a number of tumor cells (four leukemias, one mastocytoma, and one plasmacytoma) showed little or no rosette formation.

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