Effect of gonadotrophin-releasing hormone and related analogue on human luteal cell function in vitro
- 1 November 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Human Reproduction
- Vol. 4 (8) , 906-909
- https://doi.org/10.1093/oxfordjournals.humrep.a137009
Abstract
The possible direct effect of gonadotrophin-releasing hormone (GnRH) and GnRH agonist (GnRH-A; buserelin) on basal and human chorionic gonadotrophin (HCG)-stimulated progesterone (P) and cyclic AMP (cAMP) production by cultured human luteal cells was examined. Luteal cells from the early or mid-luteal phase were incubated in long-term cultures. They responded to HCG stimulation with a 2- to 3-fold increase in P production and a 2-fold increase in cAMP production. The addition of GnRH (10−7 and 10−5 M) or GnRH-A (10−7 and 10−5 M) to the medium had no effect on either basal or HCG-stimulated secretion. These results indicate that both GnRH and GnRH-A have no direct effect on human luteal steroidogenesis in vitro.Keywords
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