Electrophoretic variation of enzymes: A further marker for genetic studies of theEimeria

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Abstract
Embryo-adapted strains ofEimeria mivati andE. mivati var.diminuta, differing in their sensitivity to sulphaquinoxaline and electrophoretic mobilities of lactate dehydrogenase, were crossed.E. mivati was sulphaquinoxaline-resistant and characterised by an electrophoretic form of the enzyme denoted lactate dehydrogenase-1 whereasE. mivati var.diminuta was sulphaquinoxaline-sensitive and characterised by lactate dehydrogenase-6. Progeny recovered from the cross were passaged in embryonating eggs given sulphaquinoxaline and the (drug-resistant) parasites recovered were characterised by both lactate dehydrogenase-1 and lactate dehydrogenase-6. Controls showed that those parasites characterised by the recombinant phenotype of drug-resistance and lactate dehydrogenase-6 had been produced by the cross-fertilisation of gametes.